BAFF (Buffy-1) Monoclonal Antibody

Contact us
ProSci
Product Code: PSI-XA-1011F
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-XA-1011F-100tests100tests£729.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rat
Antibody Isotype: Rat IgG2a
Antibody Clonality: Monoclonal
Regulatory Status: RUO
Target Species: Human
Application: Flow Cytometry
Storage:
BAFF antibody can be stored at 4˚C, stable for one year.

Images

1 / 1
The immunogen is recombinant human soluble BAFF, amino acids 83-285. Anti-BAFF (MAb Buffy-1) recognizes the stalk region present in the membrane-bound form of hBAFF. Schematic structure of human soluble BAFF (rec.) (aa 83-285) as seen in this image.
❮❯

The immunogen is recombinant human soluble BAFF, amino acids 83-285. Anti-BAFF (MAb Buffy-1) recognizes the stalk region present in the membrane-bound form of hBAFF. Schematic structure of human soluble BAFF (rec.) (aa 83-285) as seen in this image.

Documents

Further Information

Application Note:
BAFF antibody detects endogenous protein by Flow Cytometry. Method: 293T cells (5 x 105) expressing surface BAFF vector or Mock transfected, were incubated on ice for 30 min in 50 μl FACS buffer (PBS, 5% fetal calf serum, 0.02% azide) containing 4 μg/mL of Buffy-1 antibody. After washing in FACS buffer, FITC-conjugated antibody to rat IgG was added and cells were analyzed by flow cytometry.
Background References:
  • BAFF, a novel ligand of the tumor necrosis factor family, stimulates B cell growth: P. Schneider, et al.; J. Exp. Med. 189, 1747 (1999).
  • BAFF binds to the tumor necrosis factor receptor-like molecule B cell maturation antigen and is important for maintaining the peripheral B cell population: J.S. Thompson, et al.; J. Exp. Med. 192, 129 (2000).
  • A soluble form of B cell maturation antigen, a receptor for the tumor necrosis factor family member APRIL, inhibits tumor cell growth: P. Rennert, et al.; J. Exp. Med. 192, 1677 (2000).
Buffer:
PBS containing 0.02% sodium azide
Concentration:
batch dependent
Conjugate:
FITC
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
BAFF (monoclonal Buffy-1) antibody was raised against recombinant human soluble BAFF (aa. 83-285).
NCBI Gene ID #:
10673
NCBI Official Name:
tumor necrosis factor (ligand) superfamily, member 13b
NCBI Official Symbol:
TNFSF13B
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Purification:
Anti BAFF (monoclonal Buffy-1) antibody was purified to ≥95% in SDS-PAGE.
Research Area:
Cytokine,Cancer
Swissprot #:
Q9Y275
User NOte:
Optimal dilutions for each application to be determined by the researcher.