CARM1 Antibody

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ProSci
Product Code: PSI-79-621
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-79-621-0.1mg0.1mg£481.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
Store antibody at -20˚C for up to one year.

Images

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Western blot analysis of lysed extracts from A431 cells untreated or treated with EGF (200 ng/mL, 5 min), using CARM1 (Ab-228) antibody (Line 1 and 2) and CARM1 (Phospho-Ser228) antibody (Line 3 and 4).
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Immunofluorescence staining of methanol-fixed HeLa cells using CARM1 (Ab-228) antibody (#21331, Red).
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Western blot analysis of lysed extracts from A431 cells untreated or treated with EGF (200 ng/mL, 5 min), using CARM1 (Ab-228) antibody (Line 1 and 2) and CARM1 (Phospho-Ser228) antibody (Line 3 and 4).
Immunofluorescence staining of methanol-fixed HeLa cells using CARM1 (Ab-228) antibody (#21331, Red).

Documents

Further Information

Additional Names:
PRMT4, PRMT4
Application Note:
Western Blot: 1:500~1:1000, Immunofluorescence: 1:100~1:200
Background:
Methylates (mono- and asymmetric dimethylation) the guanidino nitrogens of arginyl residues in several proteins involved in DNA packaging, transcription regulation, and mRNA stability. Recruited to promoters upon gene activation together with histone acetyltransferases from EP300/P300 and p160 families, methylates histone H3 at 'Arg-17' and activates transcription via chromatin remodeling. During nuclear hormone receptor activation and TCF7L2/TCF4 activation, acts synergically with EP300/P300 and either one of the p160 histone acetyltransferases NCOA1/SRC1, NCOA2/GRIP1 and NCOA3/ACTR or CTNNB1/beta-catenin to activate transcription. During myogenic transcriptional activation, acts together with NCOA3/ACTR as a coactivator for MEF2C. During monocyte inflammatory stimulation, acts together with EP300/P300 as a coactivator for NF-κ-B. Also seems to be involved in p53/TP53 transcriptional activation. Methylates EP300/P300, both at 'Arg-2142', which may loosen its interaction with NCOA2/GRIP1, and at 'Arg-580' and 'Arg-604' in the KIX domain, which impairs its interaction with CREB and inhibits CREB-dependent transcriptional activation. Also methylates arginine residues in RNA-binding proteins PABPC1, ELAVL1 and ELAV4, which may affect their mRNA-stabilizing properties and the half-life of their target mRNAs.
Background References:
  • Selma El Messaoudi, et al. (2006) Proc Natl Acad Sci U S A; 103 (36): 13351?13356. Marcela Covic, et al. (2005) EMBO J; 24 (1): 85?96. Eleni Zika et al. (2005) Proc Natl Acad Sci U S A. ; 102 (45): 16321?16326Neelu Yadav et al. (2003) Proc Natl Acad Sci U S A; 100 (11): 6464?6468
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
CARM1 (Ab-228) antibody was raised against a peptide sequence around aa.226~230 (V-K-S-N-N) derived from CARM1 .
NCBI Gene ID #:
10498
NCBI Official Name:
coactivator-associated arginine methyltransferase 1
NCBI Official Symbol:
CARM1
NCBI Organism:
This antibody was raised against a peptide sequence around aa.226~230 (V-K-S-N-N) derived from CARM1
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
63 kDa
Protein Accession #:
NP_954592.1
Protein GI Number:
40288288
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Research Area:
Other
SPECIFICITY:
This antibody detects endogenous levels of total CARM1 protein.
Swissprot #:
Q86X55