Caspase8 Antibody
| Code | Size | Price |
|---|
| PSI-79-642-0.1mg | 0.1mg | £481.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Application: Western Blot (WB)
Storage:
Store antibody at -20˚C for up to one year.
Documents
Further Information
Additional Names:
caspase-8, zgc:92075, NCAM, CASP8, MCH5
Application Note:
Western Blot: 1:500~1:1000
Background:
Most upstream protease of the activation cascade of caspases responsible for the TNFRSF6/FAS mediated and TNFRSF1A induced cell death. Binding to the adapter molecule FADD recruits it to either receptor. The resulting aggregate called death-inducing signaling complex (DISC) performs CASP8 proteolytic activation. The active dimeric enzyme is then liberated from the DISC and free to activate downstream apoptotic proteases. Proteolytic fragments of the N-terminal propeptide (termed CAP3, CAP5 and CAP6) are likely retained in the DISC. Cleaves and activates CASP3, CASP4, CASP6, CASP7, CASP9 and CASP10. May participate in the GZMB apoptotic pathways. Cleaves ADPRT. Hydrolyzes the small-molecule substrate, Ac-Asp-Glu-Val-Asp-|-AMC. Likely target for the cowpox virus CRMA death inhibitory protein. Isoform 5, isoform 6, isoform 7 and isoform 8 lack the catalytic site and may interfere with the pro-apoptotic activity of the complex.
Background References:
- Himeji D. et. al. (2002) Blood 99:4070-4078Breckenridge D. G. et. al. (2002) Proc. Natl. Acad. Sci. U. S. A. 99:4331-4336Muzio M. et. al. (1997) J. Biol. Chem. 272:2952-2956
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Caspase8 antibody was raised against a peptide sequence around aa.221~225 (T-L-D-K-V) derived from Human Caspase8.
NCBI Gene ID #:
58022
NCBI Official Name:
caspase 8, apoptosis-related cysteine peptidase
NCBI Official Symbol:
casp8
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
18, 43, 57 kDa
Protein Accession #:
NP_571585.2
Protein GI Number:
52138561
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Research Area:
Apoptosis,Cancer
SPECIFICITY:
This antibody detects endogenous level of total Caspase8 protein.
Swissprot #:
Q14790