LC3A Antibody

ProSci
Product Code: PSI-79-628
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-79-628-0.1mg0.1mg£481.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
Store antibody at -20˚C for up to one year.

Images

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Western blot analysis of lysed extracts from HeLa and 293 cells using LC3A Antibody.
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Immunofluorescence staining of methanol-fixed HeLa cells using LC3A antibody #21402.

Western blot analysis of lysed extracts from HeLa and 293 cells using LC3A Antibody.
Immunofluorescence staining of methanol-fixed HeLa cells using LC3A antibody #21402.

Further Information

Additional Names:
LC3, LC3A, ATG8E, MAP1ALC3, MAP1BLC3, Microtubule-associated protein 1 light chain 3 alpha, MAP1A/1B light chain 3 A, MAP1A/MAP1B LC3 A, MAP1 light chain 3-like protein 1, Autophagy-related protein LC3 A
Application Note:
Western Blot: 1:500~1:1000, Immunofluorescence: 1:100~1:200
Background:
Probably involved in formation of autophagosomal vacuoles (autophagosomes).
Background References:
  • Hammarback JA, et al. Neuron 7 (1): 129-39, 1991. He H, et al. J. Biol Chem 278 (31): 29278-87, 2003.
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
LC3A antibody was raised against a peptide sequence around aa.5~10 (R-P-F-K-Q) derived from Human LC3A.
NCBI Gene ID #:
84557
NCBI Official Name:
microtubule-associated protein 1 light chain 3 alpha
NCBI Official Symbol:
MAP1LC3A
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
18, 16 kDa
Protein Accession #:
NP_115903.1
Protein GI Number:
14210522
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Research Area:
Apoptosis,Cancer
SPECIFICITY:
This antibody detects endogenous level of total LC3A protein.
Swissprot #:
Q9H492