Lin28 Antibody

ProSci
Product Code: PSI-79-647
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-79-647-0.1mg0.1mg£481.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Application: Western Blot (WB)
Storage:
Store antibody at -20˚C for up to one year.

Images

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Western blot analysis of lysed extracts from HepG2 cells using Lin28 Antibody.

Western blot analysis of lysed extracts from HepG2 cells using Lin28 Antibody.

Further Information

Additional Names:
CSDD1, LIN28, LIN-28, ZCCHC1, lin-28A, CSDD1, LIN28A
Application Note:
Western Blot: 1:1000
Background:
Acts as a 'translational enhancer', driving specific mRNAs to polysomes and thus increasing the efficiency of protein synthesis. Its association with the translational machinery and target mRNAs results in an increased number of initiation events per molecule of mRNA and, indirectly, in stabilizing the mRNAs. Binds IGF2 mRNA, MYOD1 mRNA, ARBP/36B4 ribosomal protein mRNA and its own mRNA. Essential for skeletal muscle differentiation program through the translational up-regulation of IGF2 expression. Acts as a suppressor of microRNA (miRNA) biogenesis by specifically binding the precursor let-7 (pre-let-7), a miRNA precursor. Acts by binding pre-let-7 and recruiting ZCCHC11/TUT4 uridylyltransferase, leading to the terminal uridylation of pre-let-7. Uridylated pre-let-7 miRNAs fail to be processed by Dicer and undergo degradation. Degradation of pre-let-7 in embryonic stem (ES) cells contributes to the maintenance of ES cells.
Background References:
  • Heo I., Joo C., Mol. et al., Cell 32:276-284 (2008) Heo I., Joo C., Kim Y. -K., et al., Cell 138:696-708 (2009) Viswanathan S. R., Powers J. T., et al., Nat. Genet. 41:843-848 (2009)
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Lin28 antibody was raised against a peptide sequence around aa. 1~5 (M-G-S-V-S) derived from Human Lin28 .
NCBI Gene ID #:
79727
NCBI Official Name:
lin-28 homolog A (C. elegans)
NCBI Official Symbol:
LIN28A
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
26 kDa
Protein Accession #:
NP_078950.1
Protein GI Number:
13375938
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Research Area:
Stem Cell
SPECIFICITY:
This antibody detects endogenous levels of total Lin28 protein.
Swissprot #:
Q9H9Z2