MEK5 Antibody
| Code | Size | Price |
|---|
| PSI-79-696-0.1mg | 0.1mg | £481.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
- Human
- Mouse
- Rat
Application: Western Blot (WB)
Storage:
Store antibody at -20˚C for up to one year.
Documents
Further Information
Additional Names:
Mek5, MAP kinase kinase 5, Map2k5, MAPKK 5, MAPK/ERK kinase 5
Application Note:
Western Blot: 1:500
Background:
A family of protein kinases located upstream of the MAP kinases andresponsible for their activation has been identified. The prototype member ofthis family, designated MAP kinase kinase, or MEK-1, specifically phospho-rylates the MAP kinase regulatory threonine and tyrosine residues present inthe Thr-Glu-Tyr motif of ERK. A second MEK family member, MEK-2, resem-bles MEK-1 in its substrate specificity. MEK-3 (or MKK-3) functions to acti-vate p38 MAP kinase, and MEK-4 (also called SEK1 or MKK-4) activatesboth p38 and JNK MAP kinases. MEK-5 appears to specifically phosphory-late ERK5, whereas MEK-6 phosphorylates p38 and p38b. MEK-7 (or MKK-7)phosphorylates and activates the JNK signal transduction pathway.
Background References:
- Han, J., et al. 1996. J. Biol. Chem. 271: 2886-2891. Jiang, Y., et al. 1996. J. Biol. Chem. 271: 17920-17926. Tournier, C., et al. 1997. Proc. Natl. Acad. Sci. USA94: 7337-7442. Holland, P. M., et al. 1997. J. Biol. Chem. 272: 24994-24998.
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
MEK5 antibody was raised against a peptide sequence around aa. 311-315 (K-T-Y-V-G ) derived from Rat MEK5.
NCBI Gene ID #:
29568
NCBI Official Name:
mitogen activated protein kinase kinase 5
NCBI Official Symbol:
Map2k5
NCBI Organism:
This antibody was raised against a peptide sequence around aa. 311-315 (K-T-Y-V-G ) derived from Rat MEK5.
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
49 kDa
Protein Accession #:
NP_001029159.1
Protein GI Number:
77157802
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific peptide
Research Area:
Other
SPECIFICITY:
This antibody detects endogenous level of total MEK5 protein.
Swissprot #:
Q62862