PAK1/PAK2/PAK3 Antibody

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ProSci
Product Code: PSI-79-346
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-79-346-0.1mg0.1mg£481.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Store antibody at -20˚C for up to one year.

Images

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Western blot analysis of lysed extracts from MCF-7 and HeLa cells using PAK1/PAK2/PAK3 (Ab-423/402/421).
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<b>Top Image:</b> Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using PAK1/PAK2/PAK3 (Ab-423/402/421).<b>Bottom Image:</b> Immunofluorescence staining of methanol-fixed HeLa cells using PAK1/PAK2/PAK3(Ab-423/402/421).
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Western blot analysis of lysed extracts from MCF-7 and HeLa cells using PAK1/PAK2/PAK3 (Ab-423/402/421).
<b>Top Image:</b> Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using PAK1/PAK2/PAK3 (Ab-423/402/421).<b>Bottom Image:</b> Immunofluorescence staining of methanol-fixed HeLa cells using PAK1/PAK2/PAK3(Ab-423/402/421).

Documents

Further Information

Additional Names:
PAKalpha, CRSBP1, HAR, XLKD1, UNQ230/PRO263, p21-activated kinase 1, PAK-1, p65-PAK, Alpha-PAK
Application Note:
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200
Background:
The activated kinase acts on a variety of targets. Likely to be the GTPase effector that links the Rho-related GTPases to the JNK MAP kinase pathway. Activated by CDC42 and RAC1. Involved in dissolution of stress fibers and reorganization of focal complexes. Involved in regulation of microtubule biogenesis through phosphorylation of TBCB. Activity is inhibited in cells undergoing apoptosis, potentially due to binding of CDC2L1 and CDC2L2.
Background References:
  • Parsons M, et al. (2005) Mol Cell Biol ; 25:1680-1695. Jung JH, et al. (2005) J Biol Chem; 280:40025-40031. Lampugnani MG, et al. (2002) Mol Biol Cell ; 13: 1175-1189Wang RA, et al. (2002) EMBO J; 21: 5437 - 5447.
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
PAK1/PAK2/PAK3 (Ab-423/402/421) antibody was raised against a peptide sequence around aa.421~425/400~404/419~423 (R-S-T-M-V) derived from Human PAK1/PAK2/PAK3.
NCBI Gene ID #:
5058 / 5062 / 5063
NCBI Official Name:
p21 protein (Cdc42/Rac)-activated kinase 1 / p21 protein (Cdc42/Rac)-activated kinase 2 / p21 protein (Cdc42/Rac)-activated kinase 3
NCBI Official Symbol:
PAK1 PAK2 PAK3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
61 - 67 (PAK2) 68 - 74 (PAK1/3) kDa
Protein Accession #:
NP_001122092.1 / NP_002568.2 / NP_001121638.1
Protein GI Number:
190886457 / 32483399 / 189491751
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Research Area:
Apoptosis,Cancer
SPECIFICITY:
This antibody detects endogenous level of total PAK1/PAK2/PAK3 protein.
Swissprot #:
Q13153//Q13177//O75914