ATP4B Antibody

ProSci
Product Code: PSI-25-949
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-25-949-100ul100ul£662.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Canine (dog)
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
For short periods of storage (days) store at 4˚C. For longer periods of storage, store ATP4B antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.

Images

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Antibody used in WB on Transfected 293T at 0.2-1 ug/ml.

Antibody used in WB on Transfected 293T at 0.2-1 ug/ml.

Further Information

Additional Names:
ATP4B, ATP6B
Application Note:
ATP4B antibody can be used for detection of ATP4B by ELISA at 1:1562500. ATP4B antibody can be used for detection of ATP4B by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Background:
ATP4B belongs to a family of P-type cation-transporting ATPases. The gastric H+, K+-ATPase is a heterodimer consisting of a high molecular weight catalytic alpha subunit and a smaller but heavily glycosylated beta subunit. This enzyme is a proton pump that catalyzes the hydrolysis of ATP coupled with the exchange of H (+) and K (+) ions across the plasma membrane. It is also responsible for gastric acid secretion. This gene encodes the beta subunit of the gastric H+, K+-ATPase.The protein encoded by this gene belongs to a family of P-type cation-transporting ATPases. The gastric H+, K+-ATPase is a heterodimer consisting of a high molecular weight catalytic alpha subunit and a smaller but heavily glycosylated beta subunit. This enzyme is a proton pump that catalyzes the hydrolysis of ATP coupled with the exchange of H (+) and K (+) ions across the plasma membrane. It is also responsible for gastric acid secretion. This gene encodes the beta subunit of the gastric H+, K+-ATPase. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Background References:
  • Oh, J.H., (2005) Mamm. Genome 16 (12), 942-954.
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human ATP4B.
NCBI Gene ID #:
496
NCBI Official Name:
ATPase, H+/K+ exchanging, beta polypeptide
NCBI Official Symbol:
ATP4B
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
33 kDa
Protein Accession #:
NP_000696
Protein GI Number:
4557339
Purification:
Antibody is purified by peptide affinity chromatography method.
Research Area:
Membrane
Swissprot #:
P51164
User NOte:
Optimal dilutions for each application to be determined by the researcher.