MARCH5 Antibody

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ProSci
Product Code: PSI-25-835
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-25-835-100ul100ul£662.00
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Overview

Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
For short periods of storage (days) store at 4˚C. For longer periods of storage, store MARCH5 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.

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Antibody used in WB on Human Liver at 0.2-1 ug/ml.
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Antibody used in WB on Human Liver at 0.2-1 ug/ml.

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Further Information

Additional Names:
MARCH5, FLJ20445, MARCH-V, MITOL, RNF153
Application Note:
MARCH5 antibody can be used for detection of MARCH5 by ELISA at 1:1562500. MARCH5 antibody can be used for detection of MARCH5 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Background:
MARCH5 is an ubiquitin ligase of the mitochondrial outer membrane that plays a role in the control of mitochondrial morphology by regulating mitofusin-2 (MFN2) and DRP1 (DNM1L).MARCH5 is a ubiquitin ligase of the mitochondrial outer membrane that plays a role in the control of mitochondrial morphology by regulating mitofusin-2 (MFN2; MIM 608507) and DRP1 (DNM1L; MIM 603850) (Nakamura et al., 2006 [PubMed 16936636]).
Background References:
  • Karbowski, M., (2007) J. Cell Biol. 178 (1), 71-84.
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human MARCH5.
NCBI Gene ID #:
54708
NCBI Official Name:
membrane-associated ring finger (C3HC4) 5
NCBI Official Symbol:
Mar-05
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
31 kDa
Protein Accession #:
NP_060294
Protein GI Number:
8923415
Purification:
Antibody is purified by peptide affinity chromatography method.
Research Area:
Membrane
Swissprot #:
Q9NX47
User NOte:
Optimal dilutions for each application to be determined by the researcher.