CASP4 Antibody

ProSci
Product Code: PSI-45-363
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-45-363-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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45-363 (0.3ug/ml) staining of Human Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

45-363 (0.3ug/ml) staining of Human Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Further Information

Additional Names:
CASP4, TX, ICH-2, Mih1/TX, ICEREL-II, ICE(rel)II, caspase 4, apoptosis-related cysteine protease, apoptotic cysteine protease Mih1/TX, caspase 4
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 40kDa band observed in Human Heart lysates (calculated MW of 43.3kDa according to NP_001216.1). Recommended concentration: 0.3-1ug/ml.
Background References:
  • Munday NA, Vaillancourt JP, Ali A, Casano FJ, Miller DK, Molineaux SM, Yamin TT, Yu VL, Nicholson DW. Molecular cloning and pro-apoptotic activity of ICErelII and ICErelIII, members of the ICE/CED-3 family of cysteine proteases. J Biol Chem. 1995 Jun 30;270(26):15870-6.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Cat, Dog, Cow
Immunogen:
The immunogen for this antibody is: CKERAEEIYPIKERN
NCBI Gene ID #:
837
NCBI Official Name:
caspase 4, apoptosis-related cysteine peptidase
NCBI Official Symbol:
CASP4
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_001216.1, NP_150649.1
Protein GI Number:
4502577
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
This antibody is expected to recognise both isoforms of CASP4 (alpha, represented by NP_001216.1 and gamma, NP_150649.1).
Swissprot #:
P49662