COMT Antibody

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ProSci
Product Code: PSI-45-416
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-45-416-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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45-416 (0.3ug/ml) staining of Human Testis lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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45-416 (3.75ug/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
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45-416 (3.75ug/ml) staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
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45-416 (0.3ug/ml) staining of Human Testis lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
45-416 (3.75ug/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
45-416 (3.75ug/ml) staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Documents

Further Information

Additional Names:
COMT, catechol-O-methyltransferase
Application Note:
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 28kDa band in Human Testis lysates (calculated MW of 30.0kDa according to NP_000745.1 and 24.5kDa according to NP_009294.1). Recommended concentration: 0.03-0.1ug/ml.Immunohistochemistry:In paraffin embedded Human Brain (Cerebellum) shows staining of processes coming from the edge of the granular zone into the molecular layer. Recommended concentration: 2-4ug/ml. Paraffin embedded Human Testis and Small Intestine. Recommended concentration: 3.75ug/ml.Enzyme immunoassay: Sandwich-type ELISA with increasing amount of recombinant COMTcaptured by a rabbit antibody. Recommended reporter concentration: 0.5-2ug/ml.
Background References:
  • Tunbridge EM, Harrison PJ, Weinberger DR. Catechol-o-Methyltransferase, Cognition, and Psychosis: Val(158)Met and Beyond. Biol Psychiatry. 2006 Feb 11;
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
The immunogen for this antibody is: C-QDIIPQLKKKYDVD
NCBI Gene ID #:
1312
NCBI Official Name:
catechol-O-methyltransferase
NCBI Official Symbol:
COMT
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_000745.1, NP_009294.1
Protein GI Number:
4502969
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
This antibody is expected to recognise both reported isoforms (NP_000745.1 and NP_009294.1)
Swissprot #:
P21964