DKK1 Antibody
| Code | Size | Price |
|---|
| PSI-45-049-0.1mg | 0.1mg | £626.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.
Documents
Further Information
Additional Names:
DKK1, SK, DKK-1, dickkopf homolog 1 (Xenopus laevis), dickkopf-1 like, dickkopf (Xenopus laevis) homolog 1, dickkopf homolog 1, dickkopf related protein-1
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 38kDa band observed in Human Bone Marrow and Spleen lysates (calculated MW of 28.7kDa according to NP_036374.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Fedi et al, J Biol Chem. 1999 Jul 2;274(27):19465-72; PMID: 10383463). Recommended concentration: 1-3ug/ml. Primary incubation was 1 hour. Preliminary testing was unsuccessful on Pig Spleen for this particular batch.Immunohistochemistry:Paraffin embedded Human Placenta. Recommended concentration: 10ug/ml.
Background References:
- Fedi P, Bafico A, Nieto Soria A, Burgess WH, Miki T, Bottaro DP, Kraus MH, Aaronson SA. Isolation and biochemical characterization of the human Dkk-1 homologue, a novel inhibitor of mammalian Wnt signaling. J Biol Chem. 1999 Jul 2;274(27):19465-72.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Rat, Dog, Pig, Cow
Immunogen:
The immunogen for this antibody is: CDHHQASNSSRLHT
NCBI Gene ID #:
22943
NCBI Official Name:
dickkopf WNT signaling pathway inhibitor 1
NCBI Official Symbol:
DKK1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_036374.1
Protein GI Number:
7110719
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Signal Transduction
SPECIFICITY:
.
Swissprot #:
O94907