IL-1RA Antibody
| Code | Size | Price |
|---|
| PSI-38-292-0.1mg | 0.1mg | £524.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Neutralisation
- Western Blot (WB)
Storage:
The lyophilized antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-80˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repea
Documents
Further Information
Additional Names:
DIRA, IRAP, IL1F3, IL1RA, MVCD4, IL-1RN, IL-1ra, IL-1ra3, ICIL-1RA, Interleukin-1 receptor antagonist protein
Application Note:
ELISA:
Sandwich:
To detect Human IL-1RA by sandwich ELISA (using 100μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci?s Polyclonal Biotinylated Anti-Human IL-1RA (38-139) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human IL-1RA.
Western Blot:
To detect Human IL-1RA by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant Human IL-1RA is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of Human IL-1RA (50.0 ng/mL) and Human IL-1a(50.0 pg/mL), a concentration of 2.5-3.7 μg/mL of this antibody is required.
Sandwich:
To detect Human IL-1RA by sandwich ELISA (using 100μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci?s Polyclonal Biotinylated Anti-Human IL-1RA (38-139) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human IL-1RA.
Western Blot:
To detect Human IL-1RA by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant Human IL-1RA is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of Human IL-1RA (50.0 ng/mL) and Human IL-1a(50.0 pg/mL), a concentration of 2.5-3.7 μg/mL of this antibody is required.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
E.coli derived Recombinant Human IL-1Ra
NCBI Gene ID #:
3557
NCBI Official Name:
interleukin 1 receptor antagonist
NCBI Official Symbol:
IL1RN
NCBI Organism:
Homo sapiens
Physical State:
Lyophilized
Protein Accession #:
P18510
Protein GI Number:
124312
Purification:
IL-1RA specific antibody was purified by affinity chromatography employing an immobilized Human IL-1RA matrix.
Research Area:
Chemokines & Cytokines,Antibody Pairs, Functional Assays
Swissprot #:
P18510
User NOte:
Centrifuge vial prior to opening.