MIP-3 alpha Monoclonal Antibody
| Code | Size | Price |
|---|
| PSI-38-236-0.5mg | 0.5mg | £488.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Antibody Clonality: Monoclonal
Regulatory Status: RUO
Target Species: Human
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Neutralisation
- Western Blot (WB)
Storage:
MIP-3 alpha lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20˚C. Reconstitute in sterile water to a concentration of 0.1 - 1.0 mg/mL. The antibody is stable for at least six weeks a
Documents
Further Information
Additional Names:
Macrophage Inflammatory Protein 3 alpha, SCYA20, Small inducible cytokine A20, LARC, Liver and activation regulated chemokine, exodus-1, MIP3A, C-C motif chemokine 20, Beta-chemokine exodus-1, MIP-3-alpha, CKb4, ST38, MIP-3a
Application Note:
Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of mIL-10 (0.5 ng/mL), a concentration of 3.0-5.0 μg/mL of this antibody is required.
ELISA:
In a sandwich ELISA (assuming 100 μL/well), a concentration of 4.0-8.0 μg/mL of this antibody will detect at least 1600 pg/mL of recombinant murine IL-10 when used with our biotinylated antigen affinity purified anti-murine IL-10 (XP-5162Bt) as the detection antibody at a concentration of approximately 0.5-1.0 μg/mL.
Western blot:
To detect mIL-10 by Western Blot analysis this antibody can be used at a concentration of 1.0-2.0 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-10 is 2.0-4.0 ng/lane, under reducing or non-reducing conditions.
To yield one-half maximal inhibition [ND50] of the biological activity of mIL-10 (0.5 ng/mL), a concentration of 3.0-5.0 μg/mL of this antibody is required.
ELISA:
In a sandwich ELISA (assuming 100 μL/well), a concentration of 4.0-8.0 μg/mL of this antibody will detect at least 1600 pg/mL of recombinant murine IL-10 when used with our biotinylated antigen affinity purified anti-murine IL-10 (XP-5162Bt) as the detection antibody at a concentration of approximately 0.5-1.0 μg/mL.
Western blot:
To detect mIL-10 by Western Blot analysis this antibody can be used at a concentration of 1.0-2.0 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-10 is 2.0-4.0 ng/lane, under reducing or non-reducing conditions.
Background:
Macrophage Inflammatory Protein 3 alpha is a chemotactic factor that attracts lymphocytes and, slightly, neutrophils, but not monocytes. It inhibits proliferation of myeloid progenitors in colony formation assays. It binds to CCR6.
Was product number XP-5713-M
Was product number XP-5713-M
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
MIP-3 alpha antibody is produced in mice using highly pure (>98%) recombinant human MIP- 3alpha as the immunizing antigen.
NCBI Gene ID #:
6364
NCBI Official Name:
chemokine (C-C motif) ligand 20
NCBI Official Symbol:
CCL20
NCBI Organism:
Homo sapiens
Physical State:
Lyophilized
Protein Accession #:
P78556
Protein GI Number:
2829668
Purification:
This IgG1K antibody was purified from cell culture by Protein A affinity chromatography.
Research Area:
Immunology,Chemokines & Cytokines, Functional Assays
Swissprot #:
P78556
User NOte:
Centrifuge vial prior to opening.