NOTCH4 Antibody

ProSci
Product Code: PSI-42-350
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-42-350-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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42-350 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane and nuclear staining. The nuclear stain i
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42-350 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane and nuclear staining. The nuclear stain i

Further Information

Additional Names:
NOTCH4, DAQB-143M3.4, FLJ16302, INT3, MGC74442, NOTCH3, Notch homolog 4, neurogenic locus notch homolog protein 4
Application Note:
Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Preliminary experiments gave an approx 27kDa band in all human, mouse and rat tissue and cell line lysates tested after 1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 210kDa according to NP_004548.3. The 27kDa band was successfully blocked by incubation with the immunizing peptide.Immunofluorescence: Strong expression of the protein seen in the nuclei and membranes of HeLa cells, and in the membranes of NIH3T3 cells. Recommended concentration: 10ug/ml.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Rat, Dog, Pig, Cow
Immunogen:
The immunogen for this antibody is: DHFHNGHCEKGCNN
NCBI Gene ID #:
4855
NCBI Official Name:
notch 4
NCBI Official Symbol:
NOTCH4
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_004548.3
Protein GI Number:
55770876
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Transcription ,Stem Cell
SPECIFICITY:
The immunizong peptide represents part of the extracellular domain.
Swissprot #:
Q99466