MCP-5 Antibody
| Code | Size | Price |
|---|
| PSI-XP-5226-0.1mg | 0.1mg | £524.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Mouse
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Neutralisation
- Western Blot (WB)
Storage:
MCP-5 antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-
Documents
Further Information
Additional Names:
MCP-5, Scya12, Mcp5, C-C motif chemokine 12, MCP-1-related chemokine, MCP-5
Application Note:
Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of mMCP-5 (100 ng/mL), a concentration of 2.0 - 3.0 μg/mL of this antibody is required.
ELISA:
To detect mMCP-5 by direct ELISA (using 100 μg/mL of this μL/well antibody solution) a concentration of at least 0.5 antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant mMCP-5.
Sandwich:
To detect mMCP-5 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Murine MCP-5 (XP-5226Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mMCP-5.
Western Blot:
To detect mMCP-5 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mMCP-5 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
To yield one-half maximal inhibition [ND50] of the biological activity of mMCP-5 (100 ng/mL), a concentration of 2.0 - 3.0 μg/mL of this antibody is required.
ELISA:
To detect mMCP-5 by direct ELISA (using 100 μg/mL of this μL/well antibody solution) a concentration of at least 0.5 antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant mMCP-5.
Sandwich:
To detect mMCP-5 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Murine MCP-5 (XP-5226Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mMCP-5.
Western Blot:
To detect mMCP-5 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mMCP-5 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Background:
MCP-5 is a member of the b-chemokine family (C-C) of cytokines. This chemokine is strongly chemotactic for human monocytes and murine peritoneal macrophages, but is not active on mouse neutrophils and shows only a weak response on mouse eosinophils at high concentrations
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant mMCP-5 (murine MCP-5).
NCBI Gene ID #:
20293
NCBI Official Name:
chemokine (C-C motif) ligand 12
NCBI Official Symbol:
Ccl12
NCBI Organism:
Mus musculus
Physical State:
Lyophilized
Protein Accession #:
Q62401
Protein GI Number:
2493663
Purification:
Anti-mMCP-5 specific antibody was purified by affinity chromatography employing immobilized mMCP-5 matrix.
Research Area:
Chemokines & Cytokines,Antibody Pairs, Functional Assays
Swissprot #:
Q62401
User NOte:
Centrifuge vial prior to opening.