VMAT2 Antibody

ProSci
Product Code: PSI-45-146
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-45-146-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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45-146 (1ug/ml) staining of Human Placenta (A) and Human Adrenal Gland (B) lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
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45-146 Negative Control showing staining of paraffin embedded Human Adrenal Gland, with no primary antibody.

45-146 (1ug/ml) staining of Human Placenta (A) and Human Adrenal Gland (B) lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
45-146 Negative Control showing staining of paraffin embedded Human Adrenal Gland, with no primary antibody.

Further Information

Additional Names:
SLC18A2, VMAT2, SVAT, SVMT, VAT2, solute carrier family 18 (vesicular monoamine), member 2, MGC120477, MGC120478, MGC26538
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 55kDa band observed in Human Placenta and Adrenal Gland lysates (calculated MW of 55.7kDa according to NP_003045.2). Recommended concentration 1-3ug/ml. Primary incubation was 1 hour. Preliminary testing was unsuccessful on Rat Brain for this particular batch.Immunohistochemistry:Paraffin embedded Human Pancreas. Recommended concentration: 5ug/ml. In paraffin embedded Human Adrenal Gland expression of SLC18A2 is detected in chromaffin cells. Recommended concentration: 2.5ug/ml
Background References:
  • Erickson JD, Eiden LE, Hoffman BJ. Expression cloning of a reserpine-sensitive vesicular monoamine transporter. Proc Natl Acad Sci USA. 1992 Nov 15;89(22):10993-7.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Pig, Cow
Immunogen:
The immunogen for this antibody is: C-SYPIGEDEESESD
NCBI Gene ID #:
6571
NCBI Official Name:
solute carrier family 18 (vesicular monoamine transporter), member 2
NCBI Official Symbol:
SLC18A2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_003045.2
Protein GI Number:
42476325
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Neuroscience
SPECIFICITY:
.
Swissprot #:
Q05940