ZIC2 Antibody

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ProSci
Product Code: PSI-42-349
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-42-349-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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RWPE1 lysate (60ug protein in RIPA buffer) overexpressing Human ZIC2 with C-terminal MYC tag probed with 42-349 (0.5ug/ml) in the right panel and probed with anti-MYC Tag (1/1000) and anti-beta-Actin in the left panel. Mock-transfected RWPE1 in lanes 1 a
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RWPE1 lysate (60ug protein in RIPA buffer) overexpressing Human ZIC2 with C-terminal MYC tag probed with 42-349 (0.5ug/ml) in the right panel and probed with anti-MYC Tag (1/1000) and anti-beta-Actin in the left panel. Mock-transfected RWPE1 in lanes 1 a

Documents

Further Information

Additional Names:
ZIC2, Zic family member 2 (odd-paired homolog, Drosophila), HPE5, Zic family member 2 (odd-paired Drosophila homolog), Zinc finger protein of the cerebellum 2, zinc finger protein ZIC 2
Application Note:
Peptide ELISA: antibody detection limit dilution 1:1000.Western Blot:In transfected RWPE1 transiently expressing Human ZIC2-Myc a band of approx. 65kDa is observed. This band is not observed in the non-transfected RWPE1, nor when GFP is transiently expressed. The calculated molecular size is 55.0kDa according to NP_009060.2 and 58.6kDa for the ZIC2-Myc protein. Data provided by Nathan Bowen and Maryam Boseman , Clark Atlanta University, GA, USA.. We have observed an approx 75kDa band in Human Brain (Cerebellum and Cerebral Cortex) lysates Recommended concentration, 0.5-2ug/ml.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Cow
Immunogen:
The immunogen for this antibody is: C-HSGLSSNFNEWY
NCBI Gene ID #:
7546
NCBI Official Name:
Zic family member 2
NCBI Official Symbol:
ZIC2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_009060.2
Protein GI Number:
22547197
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Transcription ,Neuroscience
SPECIFICITY:
This antibody is expected to NOT cross-react with other ZIC proteins.
Swissprot #:
O95409