RFT1 Antibody
| Code | Size | Price |
|---|
| PSI-26-488-100ul | 100ul | £662.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
For short periods of storage (days) store at 4˚C. For longer periods of storage, store RFT1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Documents
Further Information
Additional Names:
RFT1, CDG1N
Application Note:
RFT1 antibody can be used for detection of RFT1 by ELISA at 1:1562500. RFT1 antibody can be used for detection of RFT1 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Background:
N-glycosylation of proteins follows a highly conserved pathway that begins with the synthesis of a??Man (5)GlcNAc (2)-dolichylpyrophosphate (PP-Dol) intermediate on the cytoplasmic side of the endoplasmic reticulum (ER) membrane followed by the translocation of Man (5)GlcNAc (2)-PP-Dol to the luminal side of the ER membrane. RFT1 is the flippase enzyme that catalyzes this translocation.N-glycosylation of proteins follows a highly conserved pathway that begins with the synthesis of a Man (5)GlcNAc (2)-dolichylpyrophosphate (PP-Dol) intermediate on the cytoplasmic side of the endoplasmic reticulum (ER) membrane followed by the translocation of Man (5)GlcNAc (2)-PP-Dol to the luminal side of the ER membrane. RFT1 is the flippase enzyme that catalyzes this translocation (Helenius et al., 2002 [PubMed 11807558]).
Background References:
- Haeuptle, M.A., (2008) Am. J. Hum. Genet. 82 (3), 600-606.
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human RFT1.
NCBI Gene ID #:
91869
NCBI Official Name:
RFT1 homolog (S. cerevisiae)
NCBI Official Symbol:
RFT1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
60 kDa
Protein Accession #:
NP_443091
Protein GI Number:
16418361
Purification:
Antibody is purified by peptide affinity chromatography method.
Research Area:
Membrane ,Cell Cycle ,Signal Transduction
Swissprot #:
Q96AA3
User NOte:
Optimal dilutions for each application to be determined by the researcher.