RPN1 Antibody

ProSci
Product Code: PSI-29-301
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-29-301-100ul100ul£662.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
For short periods of storage (days) store at 4˚C. For longer periods of storage, store RPN1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.

Images

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Antibody used in WB on HeLa , CHO-K1, rouch canine microsomes at 1 ug/ml.

Antibody used in WB on HeLa , CHO-K1, rouch canine microsomes at 1 ug/ml.

Further Information

Additional Names:
RPN1, DKFZp686B16177, OST1, RBPH1
Application Note:
RPN1 antibody can be used for detection of RPN1 by ELISA at 1:62500. RPN1 antibody can be used for detection of RPN1 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Background:
RPN1 is a type I integral membrane protein found only in the rough endoplasmic reticulum. It is part of an N-oligosaccharyl transferase complex that links high mannose oligosaccharides to asparagine residues found in the Asn-X-Ser/Thr consensus motif of nascent polypeptide chains. RPN1 forms part of the regulatory subunit of the 26S proteasome and may mediate binding of ubiquitin-like domains to this proteasome. This gene encodes a type I integral membrane protein found only in the rough endoplasmic reticulum. The encoded protein is part of an N-oligosaccharyl transferase complex that links high mannose oligosaccharides to asparagine residues found in the Asn-X-Ser/Thr consensus motif of nascent polypeptide chains. This protein forms part of the regulatory subunit of the 26S proteasome and may mediate binding of ubiquitin-like domains to this proteasome. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Background References:
  • Ewing, R.M., Mol. Syst. Biol. 3, 89 (2007).
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human RPN1.
NCBI Gene ID #:
6184
NCBI Official Name:
ribophorin I
NCBI Official Symbol:
RPN1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
66 kDa
Protein Accession #:
NP_002941
Protein GI Number:
4506675
Purification:
Antibody is purified by peptide affinity chromatography method.
Research Area:
Membrane ,RNA Binding ,Signal Transduction
Swissprot #:
P04843
User NOte:
Optimal dilutions for each application to be determined by the researcher.