Annexin A10 Antibody

ProSci
Product Code: PSI-45-255
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-45-255-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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45-255 (3.8ug/ml) staining of paraffin embedded Human Pancreas. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

45-255 (3.8ug/ml) staining of paraffin embedded Human Pancreas. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Further Information

Additional Names:
ANXA10, annexin A10, ANX14, annexin 14
Application Note:
Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Preliminary experiments (at 1ug/ml) gave an approx 45-48kDa band in Hela, A431 and Human Kidney lysates. Please note that currently we cannot find an explanation in the literature for the band we observe given the predicted size of approx. 37kDa according to NP_009124. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?Immunohistochemistry:In paraffin embedded Human Pancreas shows staining of islet of Langerhans . Recommended concentration, 3-5ug/ml.
Background References:
  • Morgan RO, Jenkins NA, Gilbert DJ, Copeland NG, Balsara BR, Testa JR, Fernandez MP. Novel human and mouse annexin A10 are linked to the genome duplications during early chordate evolution. Genomics. 1999 Aug 15;60(1):40-9.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Cow
Immunogen:
The immunogen for this antibody is: FCGDYVQGTIFPAPN
NCBI Gene ID #:
11199
NCBI Official Name:
annexin A10
NCBI Official Symbol:
ANXA10
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_009124.2
Protein GI Number:
48762937
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
.
Swissprot #:
Q9UJ72