KLK5 Antibody

ProSci
Product Code: PSI-45-809
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-45-809-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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45-809 (2ug/ml) staining of Human Brain (Cerebellum) (A), Mouse Brain (B) and Rat Brain (C) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

45-809 (2ug/ml) staining of Human Brain (Cerebellum) (A), Mouse Brain (B) and Rat Brain (C) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Further Information

Additional Names:
KLK5, kallikrein-related peptidase 5 , KLK-L2, KLKL2, SCTE , kallikrein 5, kallikrein-like protein 2, stratum corneum tryptic enzyme
Application Note:
Peptide ELISA: antibody detection limit dilution 1:1000.Western Blot:Approx 35kDa band observed in Human Brain (Cerebellum), Mouse Brain and Rat Brain lysates (calculated MW of 32.0kDa according to NP_036559.1). Recommended concentration: 1-3ug/ml.
Background References:
  • Michael IP, Pampalakis G, Mikolajczyk SD, Malm J, Sotiropoulou G, Diamandis EP. Human tissue kallikrein 5 is a member of a proteolytic cascade pathway involved in seminal clot liquefaction and potentially in prostate cancer progression. J Biol Chem. 2006 May 5;281(18):12743-50. Epub 2006 Mar 3.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Dog, Cow
Immunogen:
The immunogen for this antibody is: KAGRDSCQGD
NCBI Gene ID #:
25818
NCBI Official Name:
kallikrein-related peptidase 5
NCBI Official Symbol:
KLK5
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_036559.1
Protein GI Number:
6912644
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
Reported variants represent identical protein (NP_036559.1; NP_001070959.1; NP_001070960.1).
Swissprot #:
Q9Y337