MAP4K1 Antibody

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ProSci
Product Code: PSI-45-086
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-45-086-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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45-086 staining (0.5ug/ml) of Jurkat lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.
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45-086 staining (0.5ug/ml) of Jurkat lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.

Documents

Further Information

Additional Names:
MAP4K1, mitogen-activated protein kinase kinase kinase kinase 1, HPK1, hematopoietic progenitor kinase 1
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 95-100kDa band observed in Jurkat (predicted MW of 96kDa according to NP_009112). Recommended for use at 0.5-2ug/ml.Immunohistochemistry:Paraffin embedded Human Thymus. Recommended concentration: 2.5ug/ml.
Background References:
  • Hu MC, Qiu WR, Wang X, Meyer CF, Tan TH. Human HPK1, a novel human hematopoietic progenitor kinase that activates the JNK/SAPK kinase cascade. Genes Dev. 1996 Sep 15;10(18):2251-64.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Pig, Cow
Immunogen:
The immunogen for this antibody is: DVVDPDIFNRDPR-C
NCBI Gene ID #:
11184
NCBI Official Name:
mitogen-activated protein kinase kinase kinase kinase 1
NCBI Official Symbol:
MAP4K1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_001036065.1, NP_009112.1
Protein GI Number:
6005810
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Signal Transduction
SPECIFICITY:
This antibody is expected to recognise isoform 1 (NP_001036065.1) and isoform 2 (NP_009112.1).
Swissprot #:
Q92918