NUP84 Antibody

ProSci
Product Code: PSI-42-354
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-42-354-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Yeast
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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42-354 (2ug/ml) staining of Saccharomyces cerevisiae S288c lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Data kindly provided by Dr. F Reggiori, University of Utrecht, Netherlands

42-354 (2ug/ml) staining of Saccharomyces cerevisiae S288c lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Data kindly provided by Dr. F Reggiori, University of Utrecht, Netherlands

Further Information

Additional Names:
NUP84, Nup84p, YDL116W, Nuclear pore protein NUP84, Nucleoporin NUP84
Application Note:
Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx 75kDa band observed in S. cerevisiae wildtype lysates but not in the knock-out strain (calculated MW of 83.6kDa according to NP_010167.1). Recommended concentration: 2-4ug/ml.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
The immunogen for this antibody is: ELSPTYQTERFTK-C
NCBI Gene ID #:
851441
NCBI Official Name:
NUP84
NCBI Official Symbol:
NUP84
NCBI Organism:
Saccharomyces cerevisiae S288c
Physical State:
Liquid
Protein Accession #:
NP_010167.1
Protein GI Number:
6320087
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
SPECIFICITY:
.
Swissprot #:
P52891