PDIA3 Antibody

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ProSci
Product Code: PSI-45-108
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-45-108-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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45-108 staining (0.1ug/ml) of Daudi lysate (RIPA buffer, 35ug total protein per lane). Detected by chemiluminescence.
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45-108 staining (0.1ug/ml) of Daudi lysate (RIPA buffer, 35ug total protein per lane). Detected by chemiluminescence.

Documents

Further Information

Additional Names:
PDIA3, GRP58, ERp57, protein disulfide isomerase family A, member 3, ERp60, ERp61, GRP57, HsT17083, P58, PI-PLC, 58 kDa microsomal protein, endoplasmic reticulum P58, glucose regulated protein, 58kDa, phospholipase C-alpha, protein disulfide isomerase-associated 3, ER60, protein disulfide-isomerase A3
Application Note:
Peptide ELISA: antibody detection limit dilution 1:4000.Western Blot:Approx 60kDa band observed in lysates of cell lines NIH3T3, HepG2 and Daudi (calculated MW of 56.8kDa according to NP_005304.3). Recommended concentration: 0.1-0.3ug/ml. Primary incubation was for 1 hour.
Background References:
  • Koivunen P, Horelli-Kuitunen N, Helaakoski T, Karvonen P, Jaakkola M, Palotie A, Kivirikko KI. Structures of the human gene for the protein disulfide isomerase-related polypeptide ERp60 and a processed gene and assignment of these genes to 15q15 and 1q21. Genomics. 1997 Jun 15;42(3):397-404.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Rat, Cow
Immunogen:
The immunogen for this antibody is: C-EEKPKKKKKAQEDL
NCBI Gene ID #:
2923
NCBI Official Name:
protein disulfide isomerase family A, member 3
NCBI Official Symbol:
PDIA3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_005304.3
Protein GI Number:
21361657
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Signal Transduction
SPECIFICITY:
.
Swissprot #:
P30101