MDC Antibody
| Code | Size | Price |
|---|
| PSI-XP-5229-0.1mg | 0.1mg | £524.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Neutralisation
- Western Blot (WB)
Storage:
MDC antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-th
Documents
Further Information
Additional Names:
MDC, ABCD-1, SCYA22, STCP-1, DC/B-CK, A-152E5.1, MDC, C-C motif chemokine 22, CC chemokine STCP-1,
Application Note:
Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of hMDC (100 ng/mL), a concentration of 5.0 - 7.0 μg/mL of this antibody is required.
ELISA:
To detect hMDC by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hMDC.
Sandwich:
To detect hMDC by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human MDC (XP-5229Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hMDC.
Western Blot:
To detect hMDC by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMDC is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
To yield one-half maximal inhibition [ND50] of the biological activity of hMDC (100 ng/mL), a concentration of 5.0 - 7.0 μg/mL of this antibody is required.
ELISA:
To detect hMDC by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hMDC.
Sandwich:
To detect hMDC by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human MDC (XP-5229Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hMDC.
Western Blot:
To detect hMDC by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMDC is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Background:
The MDC gene is one of several Cys-Cys (CC) cytokine genes which are clustered on the q arm of chromosome 16.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hMDC (human Macrophage-Derived Chemokine).
NCBI Gene ID #:
6367
NCBI Official Name:
chemokine (C-C motif) ligand 22
NCBI Official Symbol:
CCL22
NCBI Organism:
Homo sapiens
Physical State:
Lyophilized
Protein Accession #:
O00626
Protein GI Number:
311033357
Purification:
Anti-hMDC specific antibody was purified by affinity chromatography employing immobilized hMDC matrix.
Research Area:
Chemokines & Cytokines,Immunology,Antibody Pairs, Functional Assays
Swissprot #:
O00626
User NOte:
Centrifuge vial prior to opening.