Resistin Monoclonal Antibody

ProSci
Product Code: PSI-38-240
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-38-240-0.5mg0.5mg£488.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Clonality: Monoclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Resistin lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20˚C. Reconstitute in sterile water to a concentration of 0.1 - 1.0 mg/mL. The antibody is stable for at least six weeks at 2

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To a sandwich ELISA (assuming 100ul/well), a concentration of 4.0-8.0 ug/ml of this antibody will detect at least 200 pg/ml of recombinant human Resistin when used with ProSci's biotinylated antigen affinity purified anti-human Resistin (XP-5261Bt) as the detection antibody at a concentration of at least 0.5-1.0 ug/ml.
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This antibody stained formalin-fixed, paraffin-embedded sections of human normal placenta. The recommended concentration is 1.0 ug/ml with a two hour incubation at room temperature. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary.

To a sandwich ELISA (assuming 100ul/well), a concentration of 4.0-8.0 ug/ml of this antibody will detect at least 200 pg/ml of recombinant human Resistin when used with ProSci's biotinylated antigen affinity purified anti-human Resistin (XP-5261Bt) as the detection antibody at a concentration of at least 0.5-1.0 ug/ml.
This antibody stained formalin-fixed, paraffin-embedded sections of human normal placenta. The recommended concentration is 1.0 ug/ml with a two hour incubation at room temperature. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary.

Further Information

Additional Names:
Adipocyte Secreted Factor, ADSF, FIZZ3, HXCP1, RSTN, UNQ407/PRO1199, Resistin, Adipose tissue-specific secretory factor, XCP1, RETN1
Application Note:
ELISA:

In a sandwich ELISA (assuming 100 μL/well), a concentration of 4.0-8.0 μg/mL of this antibody will detect at least 200 pg/mL of recombinant human Resistin when used with our biotinylated antigen affinity purified anti-human Resistin as the detection antibody at a concentration of at least 0.5-1.0 μg/mL.

Western Blot:
To detect hResistin by Western Blot analysis this antibody can be used at a concentration of 0.20-0.40 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hResistin is 0.20-0.40 ng/lane, under non-reducing conditions.
Background:
Resistin is a cytokine that is specifically secreted by adipocytes and seems to suppress the ability of insulin to stimulate glucose uptake into adipose cells. While the function of resistin is not well understood, it appears to have key paracrine roles and has a suggested role in type 2 diabetes.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Resistin antibody is produced in BALB/c mice using highly pure (>98%) recombinant human Resistin as the immunizing antigen.
NCBI Gene ID #:
56729
NCBI Official Name:
resistin
NCBI Official Symbol:
RETN
NCBI Organism:
Homo sapiens
Physical State:
Lyophilized
Protein Accession #:
Q9HD89
Protein GI Number:
18202962
Purification:
This IgG2bK antibody was purified from ascites fluid by Protein A affinity chromatography.
Research Area:
Signal Transduction,Chemokines & Cytokines
Swissprot #:
Q9HD89
User NOte:
Centrifuge vial prior to opening.