EPM2A Antibody

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ProSci
Product Code: PSI-45-547
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-45-547-0.1mg0.1mg£626.00
Quantity:
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Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Canine (dog)
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human EPM2AI with DYKDDDDK tag probed with 45-547 (1ug/ml) in Lane A and probed with anti-DYKDDDDK Tag (1/1000) in lane C. Mock-transfected HEK293 probed with 45-547 (1mg/ml) in Lane B. Primar
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HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human EPM2AI with DYKDDDDK tag probed with 45-547 (1ug/ml) in Lane A and probed with anti-DYKDDDDK Tag (1/1000) in lane C. Mock-transfected HEK293 probed with 45-547 (1mg/ml) in Lane B. Primar

Documents

Further Information

Additional Names:
EPM2AIP1, FLJ11207, KIAA0766, EPM2A (laforin) interacting protein 1, laforin interacting protein 1, EPM2A interacting protein 1
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:In transfected HEK293 transiently expressing full-length Human EPM2AIP1 (myc and DYKDDDDK tagged), a band of approx. 75kDa was observed. No bands were observed in mock-transfected HEK293 and the same band was observed using anti- DYKDDDDK tag antibody. Recommended concentration, 1-2ug/ml.
Background References:
  • Chan EM, Ackerley CA, Lohi H, Ianzano L, Cortez MA, Shannon P, Scherer SW, Minassian BA. Laforin preferentially binds the neurotoxic starch-like polyglucosans, which form in its absence in progressive myoclonus epilepsy. Hum Mol Genet. 2004 Jun 1;13(11):1117-29. Epub 2004 Apr 21.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Cow
Immunogen:
The immunogen for this antibody is: WMTPKRSKMEVDC
NCBI Gene ID #:
9852
NCBI Official Name:
EPM2A (laforin) interacting protein 1
NCBI Official Symbol:
EPM2AIP1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_055620.1
Protein GI Number:
7662294
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
Swissprot #:
Q7L775