Rabphilin Antibody

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ProSci
Product Code: PSI-50-236
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-50-236-0.1mL0.1mL£686.00
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Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Mouse
  • Rat
Applications:
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Rabphilin antibody can be stored at -20˚C and is stable at -20˚C for at least 1 year.

Images

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Western blot of rat brain lysate showing specific immunolabeling of the ~82k rabphilin 3A phosphorylated at Ser234.
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Western blot of rat brain lysate showing specific immunolabeling of the ~82k rabphilin 3A phosphorylated at Ser234.

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Further Information

Application Note:
The antibody has been directly tested for reactivity in Western blots with rat tissue. It is anticipated that the antibody will react with mouse based on the fact that this species has 100% homology with the amino acid sequence used as antigen.
Background:
Rabphilin-3A is a peripheral membrane protein that binds Ca2+ and phospholipids and is attached to synaptic vesicle membranes (Geppert et al., 1994;Li et al., 1994). The expression of rabphilin 3A is reduced in an animal model of Huntington?s disease(Smith et al., 2005). Rabphilin can be phosphorylated at Ser234 by both CAM kinase II and PKA and this phosphorylation has been suggested to regulate neuronal activity during development in a synapse-specific manner (Fykse et al., 1995;Foletti and Scheller, 2001).
Background References:
  • Foletti DL, Scheller RH (2001) Developmental regulation and specific brain distribution of phosphorabphilin. J Neurosci 21:5461-5472.
  • Fykse EM, Li C, Sudhof TC (1995) Phosphorylation of rabphilin-3A by Ca2+/calmodulin- and cAMP-dependent protein kinases in vitro. J Neurosci 15:2385-2395.
  • Geppert M, Bolshakov VY, Siegelbaum SA, Takei K, De Camilli P, Hammer RE, S?dhof TC (1994) The role of Rab3A in neurotransmitter release. Nature (Lond ) 369:493-497.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser234 of rabphilin 3A.
Modifications:
Phospho-Specific
NCBI Gene ID #:
171039
NCBI Official Name:
rabphilin 3A
NCBI Official Symbol:
Rph3a
NCBI Organism:
Rattus norvegicus
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
82
Protein Accession #:
P47709
Protein GI Number:
1350830
Purification:
Affinity Purified
Research Area:
Phospho-Specific
Swissprot #:
P47709
User NOte:
Optimal dilutions for each application to be determined by the researcher.