EphrinB Antibody

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ProSci
Product Code: PSI-50-222
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-50-222-0.1mL0.1mL£686.00
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Overview

Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Amphibian
  • Avian
  • Bovine (Cattle)
  • Canine (dog)
  • Fish
  • Human
  • Mouse
  • Rat
Application: Western Blot (WB)
Storage:
EphrinB antibody can be stored at -20˚C and is stable at -20˚C for at least 1 year.

Images

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Western blot of rat testes lysate showing specific immunolabeling of the ~46k EphrinB phosphorylated at Tyr298 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: lamda-Ptase). The blot is identical to the control except that it was incubated in lamda-Ptase (1200 units for 30 min) before being exposed to the Anti-Tyr298 EphrinB. The immunolabeling of the EphrinB band is completely eliminated by treatment with lamda-Ptase.
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Western blot of rat testes lysate showing specific immunolabeling of the ~46k EphrinB phosphorylated at Tyr298 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: lamda-Ptase). The blot is identical to the control except that it was incubated in lamda-Ptase (1200 units for 30 min) before being exposed to the Anti-Tyr298 EphrinB. The immunolabeling of the EphrinB band is completely eliminated by treatment with lamda-Ptase.

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Further Information

Application Note:
The antibody has been directly tested for reactivity in Western blots with rat tissue. It is anticipated that the antibody will react with bovine, canine, chicken, human, mouse, non-human primate, Xenopus and zebra fish based on the fact that these species have 100% homology with the amino acid sequence used as antigen.
Background:
EphrinB proteins are thought to play key roles in cellular functions as diverse as neuronal migration and blood vessel development (Flanagan and Vancerhaeghen, 1998; Dufour et al., 2003; Oike et al., 2002). EphrinB molecules expressed at the membrane surface bind to the EphB family receptors on target cells during cellto cell contact. This interaction leads to cell signaling in the target cell but also generates a reverse signal in the cell expressing EphrinB on its surface. This reverse signaling event is thought to be critical for vessel maturation and neuronal development. Importantly, tyrosine phosphorylation of EphrinB is thought to be a critical component of this reverse signaling event (Palmer et al., 2002). Recent work suggests that phosphorylation of a specific EphrinB residue (Tyr298) plays a key role in EphrinB signaling (Kalo, et al., 2001).
Background References:
  • Bong, Y.S., Park, Y.H., Lee, H.S., Mood, K., Ishimura, A. and Daar, I.O. Tyr-298 in ephrinB1 is critical for an interaction with the Grb4 adaptor protein, Biochem. J. 377:499-507 (2004).
  • Dufour, A., Seibt, J., Passante, L., Depaepe, V., Ciossek, T., Frisen, J., Kullander, K., Flanagan, J.G., Polleux, F. and Vanderhaeghen, P. Area specificity and topography of thalamocortical projections are controlled by ephrin/Eph genes, Neuron 39:453-465 (2003).
  • Flanagan, J.G. and Vanderhaeghen, P. The ephrins and Eph receptors in neural development, Annu. Rev. Neurosci. 21:309-345 (1998).
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Phosphopeptide corresponding to amino acid residues surrounding the phosphoTyr298 of Xenopus EphrinB. Note: Xenopus Tyr298 is the homolog of human, mouse and rat Tyr317 and also chicken Tyr305.
Modifications:
Phospho-Specific
NCBI Gene ID #:
396513
NCBI Official Name:
EPH receptor B2
NCBI Official Symbol:
EPHB2
NCBI Organism:
Gallus gallus
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
46
Protein Accession #:
P28693
Protein GI Number:
12230900
Purification:
Affinity Purified
Research Area:
Phospho-Specific
Swissprot #:
P28693
User NOte:
Optimal dilutions for each application to be determined by the researcher.