p44/42 MAP Kinase Antibody

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ProSci
Product Code: PSI-79-215
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-79-215-0.1mg0.1mg£488.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Store antibody at -20˚C for up to one year.

Images

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Western blot analysis of lysed extracts from 293 cells untreated or treated with UV using p44/42 MAP Kinase (Phospho-Tyr204).
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<b>Top Image:</b> Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using p44/42 MAP Kinase (Phospho-Tyr204).<b>Bottom Image:</b> Immunofluorescence staining of methanol-fixed HeLa cells showing centrosome and nuclear staining using p44/42 MAP Kinase (Phospho-Tyr204).
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Western blot analysis of lysed extracts from 293 cells untreated or treated with UV using p44/42 MAP Kinase (Phospho-Tyr204).
<b>Top Image:</b> Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using p44/42 MAP Kinase (Phospho-Tyr204).<b>Bottom Image:</b> Immunofluorescence staining of methanol-fixed HeLa cells showing centrosome and nuclear staining using p44/42 MAP Kinase (Phospho-Tyr204).

Documents

Further Information

Additional Names:
ERK1, ERT2, ERK-1, PRKM3, P44ERK1, P44MAPK, HS44KDAP, HUMKER1A, p44-ERK1, p44-MAPK, PKC2, ERK, Extracellular signal- regulated kinase 1, Insulin-stimulated MAP2 kinase, MAP kinase 1
Application Note:
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200
Background:
Involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK-1. Phosphorylates EIF4EBP1; required for initiation of translation. Phosphorylates microtubule-associated protein 2 (MAP2). Phosphorylates SPZ1.
Background References:
  • TETE HANNKEN, et al. (2000) Am Soc Nephrol 11:1387-1397Omar D. PerezNature et al. (2002) Biotechnology 20: 155 - 162 Jingui Yu, et al. (2005) Anesth Analg 101: 315-321Hironobu Ihn et al. (2000) Immunology 165: 2149-2155
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
p44/42 MAP Kinase (Phospho-Tyr204) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 204 (T-E-Y (p) -V-A) derived from Human p44/42 MAP Kinase.
Modifications:
Phospho-Specific
NCBI Gene ID #:
5595
NCBI Official Name:
mitogen-activated protein kinase 3
NCBI Official Symbol:
MAPK3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
42, 44 kDa
Protein Accession #:
NP_001035145.1
Protein GI Number:
91718897
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Research Area:
Phospho-Specific,Apoptosis,Cancer
SPECIFICITY:
This antibody detects endogenous level of p44/42 MAP Kinase only when phosphorylated at tyrosine 204.
Swissprot #:
P27361