ALOX5AP Antibody

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ProSci
Product Code: PSI-45-242
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-45-242-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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45-242 (0.1ug/ml) staining of Human U937 cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
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45-242 (3.75ug/ml) staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
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45-242 (0.1ug/ml) staining of Human U937 cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
45-242 (3.75ug/ml) staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Documents

Further Information

Additional Names:
ALOX5AP, FLAP, arachidonate 5-lipoxygenase-activating protein, MK-886-binding protein, five-lipoxygenase activating protein
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 16kDa band observed in lysates of U937 and of Peripheral Blood Mononucleocytes (calculated MW of 18.2kDa according to NP_001620.2). Recommended concentration: 0.1-0.3ug/ml. Primary incubation was 1 hour.Immunohistochemistry:In paraffin embedded Human Spleen shows staining of select splenocytes. Paraffin embedded Human Lung and Small Intestine. Recommended concentration: 3.75ug/ml.
Background References:
  • Kaaman M, Ryden M, Axelsson T, Nordstrom E, Sicard A, Bouloumie A, Langin D, Arner P, Dahlman I. ALOX5AP expression, but not gene haplotypes, is associated with obesity and insulin resistance. Int J Obes (Lond). 2006 Mar;30(3):447-52.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Dog
Immunogen:
The immunogen for this antibody is: C-HKVEHESRTQNGR
NCBI Gene ID #:
241
NCBI Official Name:
arachidonate 5-lipoxygenase-activating protein
NCBI Official Symbol:
ALOX5AP
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_001620.2, NP_001191335.1
Protein GI Number:
15718675
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
This antibody is expected to recognize both reported isoforms (NP_001620.2; NP_001191335.1).
Swissprot #:
P20292