DDX5 Antibody

Contact us
ProSci
Product Code: PSI-45-470
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-45-470-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

1 / 4
45-470 (0.5ug/ml) staining of cell lines A431(A), HeLa(B), HepG2(C), Jurkat(D) and NIH3T3 (E) lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
2 / 4
3 / 4
4 / 4
45-470 (2.5ug/ml) staining of paraffin embedded Human Breast. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. <strong>This data is from a previous batch, not on sale.</strong>
❮❯

45-470 (0.5ug/ml) staining of cell lines A431(A), HeLa(B), HepG2(C), Jurkat(D) and NIH3T3 (E) lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
45-470 (2.5ug/ml) staining of paraffin embedded Human Breast. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. <strong>This data is from a previous batch, not on sale.</strong>

Documents

Further Information

Additional Names:
DDX5, P68, HLR1, G17P1, HUMP68, DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 5 (RNA helicase, 68kDa), RNA-dependent ATPase, DEAD/H box-5 (RNA helicase, 68kD), DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 5 (RNA helicase, 68kD), DEAD (Asp-Glu-Ala-Asp) box polypeptide 5, DKFZp434E109, DKFZp686J01190, ATP-dependent RNA helicase DDX5, DEAD box-5
Application Note:
Peptide ELISA: antibody detection limit dilution 1:2000.Western Blot:Approx 70kDa band observed in nuclear lysates of cell lines NIH3T3, A431, HeLa and Jurkat (calculated MW of 69.1kDa according to NP_004387.1). Recommended concentration: 0.3-1ug/ml. Primary incubation was 1 hour.Immunofluorescence: A customer has reported successful staining at a dilution of 1:100 on HT-1080 cells with a Rhodamine conjugated secondary antibody. This data is from a previous batch, not on sale.
Background References:
  • Iggo R, Gough A, Xu W, Lane DP, Spurr NK. Chromosome mapping of the human gene encoding the 68- kDa nuclear antigen (p68) by using the polymerase chain reaction. Proc Natl Acad Sci USA. 1989 Aug;86(16):6211-4.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Rat, Dog
Immunogen:
The immunogen for this antibody is: C-PMIGYPMPTGYSQ
NCBI Gene ID #:
1655
NCBI Official Name:
DEAD (Asp-Glu-Ala-Asp) box helicase 5
NCBI Official Symbol:
DDX5
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_004387.1
Protein GI Number:
4758138
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer ,Infectious Disease
SPECIFICITY:
.
Swissprot #:
P17844