KLF4 Antibody

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ProSci
Product Code: PSI-45-806
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-45-806-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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45-806 (0.1ug/ml) staining of Human Duodenum lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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45-806 (0.1ug/ml) staining of Human Duodenum lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

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Further Information

Additional Names:
KLF4, GKLF, Kruppel-like factor 4 (gut), EZF, endothelial Kruppel-like zinc finger protein
Application Note:
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Experiments gave strong bands at 75-90kDa and a faint 50-kDa band in Human Duodenal lysate after 0.1ug/ml antibody staining. These bands correspond to earlier findings in literature (Piccinni et al, J. Biol. Chem., Vol. 279 (10), 9103-9114). All these bands were successfully blocked by incubation with the immunizing peptide. Recommended concentration: 0.1-0.3ug/ml.
Background References:
  • Shields JM, Christy RJ, Yang VW. Identification and characterization of a gene encoding a gut-enriched Kruppel-like factor expressed during growth arrest. J Biol Chem. 1996 Aug 16;271(33):20009-17.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Cow
Immunogen:
The immunogen for this antibody is: AVSDALLPSFST-C
NCBI Gene ID #:
9314
NCBI Official Name:
Kruppel-like factor 4 (gut)
NCBI Official Symbol:
KLF4
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_004226.2
Protein GI Number:
194248077
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
.
Swissprot #:
O43474