APPL1 Antibody

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ProSci
Product Code: PSI-45-271
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-45-271-0.1mg0.1mg£626.00
Quantity:
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Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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45-271 (0.5ug/ml) staining of 1) Rat Aortic Smooth Muscle cell lysate 2) after infection with Human APPL1 adenovirus for 48hrs. Detected by chemiluminescence. Data kindly provided by Dr. L. Jia, Ann Arbor, USA.
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45-271 (0.5ug/ml) staining of 1) Rat Aortic Smooth Muscle cell lysate 2) after infection with Human APPL1 adenovirus for 48hrs. Detected by chemiluminescence. Data kindly provided by Dr. L. Jia, Ann Arbor, USA.

Documents

Further Information

Additional Names:
APPL1, DIP13alpha , adaptor protein containing pH domain, PTB domain and leucine zipper motif, signaling adaptor protein DIP13alpha
Application Note:
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 80kDa band observed in Rat Aortic Smooth Skeletal Muscle Cells (lane 1), intensity increased after infection with Human APPL1 adenovirus for 48h (lane 2). Calculated MW of 79.7kDa according to NP_036228.1. The blot was co-labeled with Tubulin-specific antibodies as a loading control. Recommended concentration: 0.3-1ug/ml.
Background References:
  • Mao X, Kikani CK, Riojas RA, Langlais P, Wang L, Ramos FJ, Fang Q, Christ-Roberts CY, Hong JY, Kim RY, Liu F, Dong LQ. APPL1 binds to adiponectin receptors and mediates adiponectin signalling and function. Nat Cell Biol. 2006 May;8(5):516-23. Epub 2006 Apr 16. Erratum in: Nat Cell Biol. 2006 Jun;8(6):642.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Human, Mouse, Rat, Dog, Cow
Immunogen:
The immunogen for this antibody is: C-NRYSRLSKKRENDK
NCBI Gene ID #:
26060
NCBI Official Name:
adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 1
NCBI Official Symbol:
APPL1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_036228.1
Protein GI Number:
6912242
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
.
Swissprot #:
Q9UKG1