ZP3 Antibody
| Code | Size | Price |
|---|
| PSI-28-372-100ul | 100ul | £609.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
For short periods of storage (days) store at 4˚C. For longer periods of storage, store ZP3 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Documents
Further Information
Additional Names:
ZP3, ZPC, ZP3A, ZP3B, Zp-3
Application Note:
ZP3 antibody can be used for detection of ZP3 by ELISA at 1:62500. ZP3 antibody can be used for detection of ZP3 by western blot at 0.6 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Background:
The zona pellucida is an extracellular matrix that surrounds the oocyte and early embryo. It is composed primarily of three or four glycoproteins with various functions during fertilization and preimplantation development. The protein encoded by this gene is a structural component of the zona pellucida and functions in primary binding and induction of the sperm acrosome reaction. The nascent protein contains a N-terminal signal peptide sequence, a conserved ZP domain, a C-terminal consensus furin cleavage site, and a transmembrane domain. It is hypothesized that furin cleavage results in release of the mature protein from the plasma membrane for subsequent incorporation into the zona pellucida matrix. However, the requirement for furin cleavage in this process remains controversial based on mouse studies. A variation in the last exon of this gene has previously served as the basis for an additional ZP3 locus; however, sequence and literature review reveals that there is only one full-length ZP3 locus in the human genome. Another locus encoding a bipartite transcript designated POMZP3 contains a duplication of the last four exons of ZP3, including the above described variation, and maps closely to this gene
Background References:
- Chamberlin, M.E., et al., (1990) Proc. Natl. Acad. Sci. U.S.A. 87 (16), 6014-6018.
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human ZP3.
NCBI Gene ID #:
7784
NCBI Official Name:
zona pellucida glycoprotein 3 (sperm receptor)
NCBI Official Symbol:
ZP3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
47 kDa, 23 kDa
Protein Accession #:
P21754-2
Protein GI Number:
251757420
Purification:
Antibody is purified by protein A chromatography method.
Research Area:
Transcription
Swissprot #:
P21754-2
User NOte:
Optimal dilutions for each application to be determined by the researcher.