Chymase 2 Antibody
| Code | Size | Price |
|---|
| PSI-46-865-0.1mg | 0.1mg | £505.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.
Documents
Further Information
Additional Names:
Cma2, Chymase 2, mast cell, Mast cell protease 10, Mcp10,
Application Note:
Peptide ELISA: antibody detection limit dilution 1:2000.Western Blot:Preliminary experiments gave an approx 49kDa band in Human Skeletal Muscle lysates after 0.02ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 26.9kDa according to NP_001019885.1 and NP_034912.3. The 49kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
Background References:
- Lee M, Calabresi L, Chiesa G, Franceschini G, Kovanen PT. Mast cell chymase degrades apoE and apoA-II in apoA-I-knockout mouse plasma and reduces its ability to promote cellular cholesterol efflux. Arterioscler Thromb Vasc Biol. 2002 Sep 1;22(9):1475-81.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse
Immunogen:
The immunogen for this antibody is: CKIFKHYKDSLQ
NCBI Gene ID #:
17232 (mouse);
NCBI Official Name:
mast cell protease 9
NCBI Official Symbol:
Cma2
NCBI Organism:
Mus musculus
Physical State:
Liquid
Protein Accession #:
NP_001019885.1, NP_034912.3
Protein GI Number:
67010053
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Homeostasis
SPECIFICITY:
Mcp 10 and Mcp 9 (GeneID 545055) are almost identical in their protein sequence. No cross-reactivity expected with the similar Mcp4.
Swissprot #:
O35164