CNN2 Antibody

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ProSci
Product Code: PSI-42-116
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-42-116-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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42-116 (0.03ug/ml) staining of HepG2 lysates (35ug protein in RIPA buffer). Detected by chemiluminescence.
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42-116 (0.03ug/ml) staining of HepG2 lysates (35ug protein in RIPA buffer). Detected by chemiluminescence.

Documents

Further Information

Additional Names:
CNN2, calponin 2, calponin H2, smooth muscle, neutral calponin
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 35kDa band observed in lysates of cell line HepG2 (calculated MW of 33.7kDa according to NP_004359.1). Recommended concentration: 0.03-0.1ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Placenta. Recommended concentration: 2.5ug/ml.
Background References:
  • Masuda H, Tanaka K, Takagi M, Ohgami K, Sakamaki T, Shibata N, Takahashi K, Molecular cloning and characterization of human non-smooth muscle calponin. Journal of biochemistry 1996 Aug 120 (2): 415-24.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
The immunogen for this antibody is: C-DPGEVPEYPPYYQEE
NCBI Gene ID #:
1265
NCBI Official Name:
calponin 2
NCBI Official Symbol:
CNN2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_004359.1, NP_958434.1
Protein GI Number:
4758018
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Signal Transduction
SPECIFICITY:
This antibody is expected to recognize both reported isoforms (NP_004359.1; NP_958434.1), but it is not expected to cross-react with CCN3.
Swissprot #:
Q99439