Cyb5r3 Antibody

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ProSci
Product Code: PSI-42-120
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-42-120-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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42-120 (0.1ug/ml) staining of Mouse Liver lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
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42-120 (0.1ug/ml) staining of Mouse Liver lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Documents

Further Information

Additional Names:
Cyb5r3, cytochrome b5 reductase 3, 0610016L08Rik, 2500002N19Rik, C85115, Dia-1, Dia1, WU:AL591952.1-001, WU:AL591952.1-002, WU:AL591952.1-003, WU:Cyb5r3, NADH-cytochrome b5 reductase, diaphorase 1, diaphorase 1 (NADH)
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 35kDa band observed in Mouse Liver lysates as well as in mouse and rat brain lysates (calculated MW of 34.1kDa according to mouse NP_084063.1). Recommended concentration: 0.1-1ug/ml.
Background References:
  • Fernandez-Borja M, Janssen L, Verwoerd D, Hordijk P, Neefjes J, RhoB regulates endosome transport by promoting actin assembly on endosomal membranes through Dia1. J. Cell. Sci. 2005 Jun 118 (Pt 12): 2661-70.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Rat, Dog, Cow
Immunogen:
The immunogen for this antibody is: PNLERVGHPKERC
NCBI Gene ID #:
109754 (mouse); 25035 (rat);
NCBI Official Name:
cytochrome b5 reductase 3
NCBI Official Symbol:
Cyb5r3
NCBI Organism:
Mus musculus
Physical State:
Liquid
Protein Accession #:
NP_084063.1
Protein GI Number:
19745150
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Signal Transduction
SPECIFICITY:
.
Swissprot #:
Q9DCN2