EIF5A Antibody

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ProSci
Product Code: PSI-42-119
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-42-119-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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42-119 (0.1ug/ml) staining of MOLT4 lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
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42-119 (3.75ug/ml) staining of paraffin embedded Human Vessel. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
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42-119 (0.1ug/ml) staining of MOLT4 lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
42-119 (3.75ug/ml) staining of paraffin embedded Human Vessel. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Documents

Further Information

Additional Names:
EIF5A, eukaryotic translation initiation factor 5A, EIF-5A, EIF5A1, MGC104255, MGC99547, eIF5AI, OTTHUMP00000128384, uORF A
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 20kDa band observed in MOLT4 lysates (calculated MW of 20.2kDa according to NP_001137232.1). Recommended concentration: 1-3ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Spleen, Tonsil and Vessel. Recommended concentration: 3.75ug/ml.
Background References:
  • Koettnitz K, Kappel B, Baumruker T, Hauber J, Bevec D The genomic structure encoding human initiation factor eIF-5A Gene. 1994 Jul 144 (2): 249-52
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
The immunogen for this antibody is: C-RPPHRASFLKRLESK
NCBI Gene ID #:
1984
NCBI Official Name:
eukaryotic translation initiation factor 5A
NCBI Official Symbol:
EIF5A
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_001137232.1
Protein GI Number:
219555707
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Homeostasis
SPECIFICITY:
Reported variants NP_001137233.1, NP_001961.1 and NP_001137234.1 represent identical protein:
Swissprot #:
P63241