HOXD10 Antibody

ProSci
Product Code: PSI-46-955
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-46-955-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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46-955 (0.2ug/ml) staining of Human Kidney lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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46-955 (0.2ug/ml) staining of Human Kidney lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Further Information

Additional Names:
HOXD10, homeobox D10, HOX4, HOX4D, HOX4E, Hox-4.4, homeo box 4D, homeo box D10
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx. 37 kDa band observed in Human Kidney lysates (calculated MW of 38.4 kDa according to NP_002139.2). Recommended concentration: 0.2-0.6ug/ml. Additional bands of unknown identity were also consistently observed at 90+26 kDa. These bands were successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?Immunohistochemistry:In paraffin embedded Human Kidney shows nuclear staining in some epithelial cells of PCT. Recommended concentration, 3-5ug/ml.
Background References:
  • Myers C, Charboneau A, Cheung I, Hanks D, Boudreau N. Sustained expression of homeobox D10 inhibits angiogenesis. Am J Pathol. 2002 Dec;161(6):2099-109.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Horse, Cow
Immunogen:
The immunogen for this antibody is: PNRSCRIEQPVTQQ
NCBI Gene ID #:
3236
NCBI Official Name:
homeobox D10
NCBI Official Symbol:
HOXD10
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_002139.2
Protein GI Number:
23510366
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Homeostasis
SPECIFICITY:
.
Swissprot #:
P28358