ICOS Antibody

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ProSci
Product Code: PSI-42-123
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-42-123-0.1mg0.1mg£626.00
Quantity:
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Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Application: Enzyme-Linked Immunosorbent Assay (ELISA)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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42-123 (2ug/ml) staining of paraffin embedded Human Lymph Node. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining.
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42-123 (3.75ug/ml) staining of paraffin embedded Human Thymus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
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42-123 (2ug/ml) staining of paraffin embedded Human Lymph Node. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining.
42-123 (3.75ug/ml) staining of paraffin embedded Human Thymus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Documents

Further Information

Additional Names:
ICOS, inducible T-cell co-stimulator, AILIM, CD278, MGC39850, activation-inducible lymphocyte immunomediatory molecule, inducible costimulator
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Preliminary experiments gave an approx 13kDa band in Human Peripheral Blood Lymphocyte lysates after 0.01ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 22.6kDa according to NP_036224.1. The 13kDa band was successfully blocked by incubation with the immunizing peptide.Immunohistochemistry:In paraffin embedded Human Lymph Node shows membrane staining just outside the mantle zone of the Germinal Centre. Recommended concentration: 1-2ug/ml. Paraffin embedded Human Tonsil and Thymus. Recommended concentration: 3.75ug/ml.
Background References:
  • Strauss L, Bergmann C, Szczepanski MJ, Lang S, Kirkwood JM, Whiteside TL, Expression of ICOS on human melanoma-infiltrating CD4+CD25highFoxp3+ T regulatory cells: implications and impact on tumor-mediated immune suppression. J Immunol. 2008 Mar 1;180(5):2967-80.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
The immunogen for this antibody is: KGGQILCDLTKTKGS
NCBI Gene ID #:
29851
NCBI Official Name:
inducible T-cell co-stimulator
NCBI Official Symbol:
ICOS
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_036224.1
Protein GI Number:
15029518
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Immunology
Swissprot #:
Q9Y6W8