NFATC2 Antibody

ProSci
Product Code: PSI-42-124
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-42-124-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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42-124 (0.3ug/ml) staining of MOLT4 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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42-124 (0.3ug/ml) staining of MOLT4 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Further Information

Additional Names:
NFATC2, nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 2, KIAA0611, NFAT1, NFATP, NFAT pre-existing subunit, NFAT transcription complex, preexisting component, OTTHUMP00000031291, T cell transcription factor NFAT1, nuclear factor of activated T-cells, cytoplasmic 2, nuclear factor of activated T-cells, preexisting component, preexisting nuclear factor of activated T-cells 2
Application Note:
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 110kDa band observed in Human lysates of cell line MOLT4 (calculated MW of 100kDa according to NP_775114.1). Recommended concentration: 0.3-1ug/ml.Immunohistochemistry:In paraffin embedded Human Spleen shows nuclear membrane and weaker cytoplasm staining in select splenocytes. Recommended concentration, 3-6ug/ml.
Background References:
  • Celil Aydemir AB, Lee S, Won Kim D, Gardner TR, Prince D, Mok Ahn J, Young-In Lee F, Nuclear factor of activated T cell mediates proinflammatory gene expression in response to mechanotransduction. Ann. N. Y. Acad. Sci. 2007 Nov 1117 : 138-42.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Cow
Immunogen:
The immunogen for this antibody is: C-QQFRTGLSSPDARYQ
NCBI Gene ID #:
4773
NCBI Official Name:
nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 2
NCBI Official Symbol:
NFATC2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_036472.2, NP_775114.1, NP_001129493.1
Protein GI Number:
27886539
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Signal Transduction
SPECIFICITY:
This antibody is expected to recognize all three reported isoforms (NP_036472.2; NP_775114.1; NP_001129493.1).
Swissprot #:
Q13469