PPP2R5A Antibody

ProSci
Product Code: PSI-46-210
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-46-210-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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46-210 staining (0.5ug/ml) of human muscle lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
2 / 2

46-210 staining (0.5ug/ml) of human muscle lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Further Information

Additional Names:
PPP2R5A, protein phosphatase 2, regulatory subunit B (B56), alpha isoform, MGC131915 , PP2A, B subunit, B' alpha isoform, PP2A, B subunit, B56 alpha isoform, PP2A, B subunit, PR61 alpha isoform, PP2A, B subunit, R5 alpha isoform, serine/threonine protein phosphatase 2A, 56 kDa regulatory subunit, alpha isoform, protein phosphatase 2, regulatory subunit B', alpha isoform, B56A, MGC131915, PR61A
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 55kDa band observed in human heart and skeletal muscle lysate (calculated MW of 56.2kDa according to NP_006234.1). Recommended for use at 0.5-2ug/ml.Immunohistochemistry:In paraffin embedded Human Heart shows textured cytoplasm staining in cardiomyocytes. Recommended concentration, 5-10ug/ml.
Background References:
  • McCright B, Virshup DM. Identification of a new family of protein phosphatase 2A regulatory subunits. J Biol Chem 1995 Nov 3;270(44):26123-8
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Rat, Dog, Pig, Cow
Immunogen:
The immunogen for this antibody is: C-AYNMHSILSNTSAE
NCBI Gene ID #:
5525
NCBI Official Name:
protein phosphatase 2, regulatory subunit B', alpha
NCBI Official Symbol:
PPP2R5A
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_006234.1
Protein GI Number:
5453950
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
.
Swissprot #:
Q15172