SET Antibody

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ProSci
Product Code: PSI-46-360
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-46-360-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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46-360 (0.3ug/ml) staining of Human Kidney lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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46-360 (3.75ug/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. <strong>This data is from a previous batch, not on sale.</strong>
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46-360 (0.3ug/ml) staining of Human Kidney lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
46-360 (3.75ug/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. <strong>This data is from a previous batch, not on sale.</strong>

Documents

Further Information

Additional Names:
SET, SET nuclear oncogene, 2PP2A, I2PP2A, IGAAD, IPP2A2, PHAPII, TAF-I, TAF-IBETA, LA-DR-associated protein II, OTTHUMP00000022286, SET translocation (myeloid leukemia-associated), Template-Activating Factor-I, chromatin remodelling factor, inhibitor of granzyme A-activated DNase, inhibitor-2 of protein phosphatase-2A, protein phosphatase type 2A inhibitor, template-activating factor I, HLA-DR-associated protein II
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 38kDa band observed in lysates of Human Kidney lysates and of cell lines Daudi and Molt4 (calculated MW of 32.1kDa according to NP_003002.2). Recommended concentration: 0.3-1ug/ml.
Background References:
  • Ozbun LL, You L, Kiang S, Angdisen J, Martinez A, Jakowlew SB. Identification of differentially expressed nucleolar TGF-beta1 target (DENTT) in human lung cancer cells that is a new member of the TSPY/SET/NAP-1 superfamily. Genomics 2001 Apr 15;73(2):179-93
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Cow
Immunogen:
The immunogen for this antibody is: SAPAAKVSKKELNS-C
NCBI Gene ID #:
6418
NCBI Official Name:
SET nuclear oncogene
NCBI Official Symbol:
SET
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_003002.2
Protein GI Number:
1263308
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
This antibody is expected to recognize isoform 2 (NP_003002.2).
Swissprot #:
Q01105