SH2D1A Antibody

ProSci
Product Code: PSI-46-366
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-46-366-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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46-366 (0.2ug/ml) staining of human lymph node lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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46-366 (0.2ug/ml) staining of human lymph node lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Further Information

Additional Names:
SH2D1A, SH2 domain protein 1A, Duncan's disease (lymphoproliferative syndrome), HGNC:10820, DSHP, EBVS, IMD5, LYP, MTCP1, SAP, XLP, XLPD, SH2 domain protein 1A, SLAM (signaling lymphocyte activation molecule) associated protein, T cell signal transduction molecule SAP, FLJ18687, RP5-1052M9.3, FLJ92177, Duncan's disease, SLAM-associated protein, T cell signal transduction molecule SAP, signaling lymphocyte activation molecule-associated protein
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 16kDa band observed in Human Lymph Node lysates (calculated MW of 14.2kDa according to NP_002342.1). In transfected HEK293 transiently expressing SH2D1A a band of approx. 17kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.2-0.6ug/ml.Immunoprecipitation:This antibody was deemed fit for IP under native conditions (observations from anonymous customer).
Background References:
  • Romero X, Benitez D, March S, Vilella R, Miralpeix M, Engel P. Differential expression of SAP and EAT-2-binding leukocyte cell-surface molecules CD84, CD150 (SLAM), CD229 (Ly9) and CD244 (2B4). Tissue Antigens. 2004 Aug;64(2):132-44.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Pig,
Immunogen:
The immunogen for this antibody is: C-QYPVEKKSSARSTQ
NCBI Gene ID #:
4068
NCBI Official Name:
SH2 domain containing 1A
NCBI Official Symbol:
SH2D1A
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_002342.1, NP_001108409.1
Protein GI Number:
4506923
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
.
Swissprot #:
O60880