UBE2L3 Antibody

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ProSci
Product Code: PSI-45-165
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-45-165-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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45-165 staining (0.5ug/ml) of HeLa lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
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45-165 (0.01ug/ml) staining of HEK293 (A), HepG2 (B) and Jurkat (C) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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Immunofluorescence staining of MCF7 (A) and HeLa (B) cells with 5ug/ml 45-165 antibody. Detected with Rabbit anti-goat IgG-Alexafluor488 antibody at 1:1000. Nuclei Counterstained with DAPI.
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45-165 staining (0.5ug/ml) of HeLa lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
45-165 (0.01ug/ml) staining of HEK293 (A), HepG2 (B) and Jurkat (C) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunofluorescence staining of MCF7 (A) and HeLa (B) cells with 5ug/ml 45-165 antibody. Detected with Rabbit anti-goat IgG-Alexafluor488 antibody at 1:1000. Nuclei Counterstained with DAPI.

Documents

Further Information

Additional Names:
UBE2L3, ubiquitin-conjugating enzyme E2L 3, UBCH7, E2-F1, L-UBC, UbcM4, ubiquitin carrier protein, ubiquitin-conjugating enzyme UBCH7, ubiquitin-protein ligase
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 18kDa band observed in lysates of cell lines HeLa HEK293, HepG2 and Jurkat and also in lysates of Human, Mouse and Rat Heart, Skeletal Muscle and Testis and of Pig Heart (calculated MW of 17.9kDa according to Human NP_003338.1 and Mouse NP_033482.1). Recommended concentration: 1-3ug/ml.Immunofluorescence: Strong expression of the protein seen in the cytoplasm. Dividing cells appear to show enhanced expression. Recommended concentration: 5ug/ml.
Background References:
  • Nuber U, Schwarz S, Kaiser P, Schneider R, Scheffner M. Cloning of human ubiquitin-conjugating enzymes UbcH6 and UbcH7 (E2-F1) and characterization of their interaction with E6-AP and RSP5. J Biol Chem. 1996 Feb 2;271(5):2795-800.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Cow
Immunogen:
The immunogen for this antibody is: C-EFTKKYGEKRPVD
NCBI Gene ID #:
7332
NCBI Official Name:
ubiquitin-conjugating enzyme E2L 3
NCBI Official Symbol:
UBE2L3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_003338.1
Protein GI Number:
4507789
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Homeostasis
SPECIFICITY:
.
Swissprot #:
P68036