ACOX2 Antibody

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ProSci
Product Code: PSI-45-194
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-45-194-0.1mg0.1mg£626.00
Quantity:
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Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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45-194 (0.5ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Primary incubation was 1 hour. Detected by chemiluminescence.
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45-194 (0.5ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Primary incubation was 1 hour. Detected by chemiluminescence.

Documents

Further Information

Additional Names:
ACOX2, acyl-Coenzyme A oxidase 2, branched chain , BCOX, BRCACOX, BRCOX, THCCox , Peroxisomal branched chain acyl-CoA oxidase, THCA-CoA oxidase, Trihydroxycoprostanoyl-CoA oxidase
Application Note:
Peptide ELISA: antibody detection limit dilution 1:4000.Western Blot:Approx 75kDa band observed in Human Liver lysates (calculated MW of 76.8kDa according to NP_003491.1). Recommended concentration: 0.5-1.5ug/ml. An additional band of 37kDa was consistently observed, however this band was not blocked by the immunizing peptide and it is therefore a non-specific signal. We call for caution when used for other assays than Western blot.
Background References:
  • Baumgart E, Vanhooren JC, Fransen M, Marynen P, Puype M, Vandekerckhove J, Leunissen JA, Fahimi HD, Mannaerts GP, van Veldhoven PP. Molecular characterization of the human peroxisomal branched-chain acyl-CoA oxidase: cDNA cloning, chromosomal assignment, tissue distribution, and evidence for the absence of the protein in Zellweger syndrome. Proc Natl Acad Sci USA. 1996 Nov 26;93(24):13748-53.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
The immunogen for this antibody is: C-QWAQKSPTNTQENP
NCBI Gene ID #:
8309
NCBI Official Name:
acyl-CoA oxidase 2, branched chain
NCBI Official Symbol:
ACOX2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_003491.1
Protein GI Number:
4501869
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
.
Swissprot #:
Q99424