POLL Antibody

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ProSci
Product Code: PSI-46-207
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-46-207-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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46-207 (5ug/ml) staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. <strong>This data is from a previous batch, not on sale.</strong>
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46-207 (5ug/ml) staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. <strong>This data is from a previous batch, not on sale.</strong>

Documents

Further Information

Additional Names:
POLL, BETA-N, POL-KAPPA, polymerase (DNA directed), lambda, polymerase (DNA-directed), lambda, DNA polymerase kappa (DNA polymerase beta-N), RP11-529I10.1, BETAN, FLJ46002, POLKAPPA, DNA polymerase beta-2, DNA polymerase kappa (DNA polymerase beta-N), DNA polymerase lamda2, DNA-directed DNA polymerase lambda, OTTHUMP00000020323, pol beta2, pol lambda
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 60kDa band observed in cell lysates of A549 and approx 60-70kDa in cell lysates of HepG2 (calculated MW of 63.5kDa according to Human NP_037406.1). Recommended concentration: 0.5-1ug/ml.
Background References:
  • Garcia-Diaz M, Bebenek K, Sabariegos R, Dominguez O, Rodriguez J, Kirchhoff T, Garcia-Palomero E, Picher AJ, Juarez R, Ruiz JF, Kunkel TA, Blanco L. DNA polymerase lambda, a novel DNA repair enzyme in human cells. J Biol Chem. 2002 Apr 12;277(15):13184-91.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
The immunogen for this antibody is: C-LGLPYREPAERDW
NCBI Gene ID #:
27343
NCBI Official Name:
polymerase (DNA directed), lambda
NCBI Official Symbol:
POLL
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_037406.1
Protein GI Number:
7019491
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
It has been brought to our attention that this product may crossreact with Pol beta (NP_002681), an evolutionarilly related polymerase with a predicted size of 39 kDa. The overall % of identity is not very high (about 32% in the core) but considering only the peptide that we used as immunogen the two share the sequence YREP**R** at the C-terminus. Further the protein structures appear to be very similar in this region.
Swissprot #:
Q9UGP5