RAB11FIP1 Antibody

ProSci
Product Code: PSI-46-257
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-46-257-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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46-257 (0.2ug/ml) staining of HeLa lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

46-257 (0.2ug/ml) staining of HeLa lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Further Information

Additional Names:
RAB11FIP1, RAB11 family interacting protein 1 (class I) , HGNC:30265, FLJ22524, FLJ22622, MGC78448, NOEL1A, RCP, rab11-FIP1, Rab coupling protein, Rab effector protein, Rab-interacting recycling protein, Rab11-family interacting protein 1D, no Rab11-binding protein 1A , DKFZp686E2214, RAB11 coupling protein, RAB11 family interacting protein 1
Application Note:
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 80kDa band observed in lysates of cell line HeLa (calculated MW of 71.0kDa according to NP_079427.3). The observed molecular weight of 80kDa corresponds to earlier findings in literature with different antibodies (Lindsay et al, J Biol Chem. 2002 Apr 5;277(14):12190-9. PMID 11786538.). Recommended concentration: 0.1-0.3ug/ml.
Background References:
  • Garcia MJ, Pole JC, Chin SF, Teschendorff A, Naderi A, Ozdag H, Vias M, Kranjac T, Subkhankulova T, Paish C, Ellis I, Brenton JD, Edwards PA, Caldas C. A 1 Mb minimal amplicon at 8p11-12 in breast cancer identifies new candidate oncogenes. Oncogene. 2005 Aug 4;24(33):5235-45.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
The immunogen for this antibody is: C-EATKEAKESKKPE
NCBI Gene ID #:
80223
NCBI Official Name:
RAB11 family interacting protein 1 (class I)
NCBI Official Symbol:
RAB11FIP1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_079427.3, NP_001002814.1
Protein GI Number:
50363228
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
This antibody is expected to recognise isoform 1 (NP_079427.3) and isoform 3 (NP_001002814.1).
Swissprot #:
Q6WKZ4